Phylogenetic tools and ‘tree-thinking’ approaches increasingly permeate biological research. At the same time phylogenetic data sets are expanding at breakneck pace, facilitated by increasingly economical sequencing technologies.Therefore, there is an increasing need for accessible, modular, and sharable tools for phylogenetic analysis. In the Oakley Lab at UCSB, we are developing Osiris, a suite of tools and workflows for the Galaxy workflow management system. We are developing Galaxy wrappers for existing software to accomplish all aspects of phylogenetic analysis, including de novo assembly of next-generation sequencing reads, ortholog identification, multiple sequence alignment, concatenation, phylogenetic tree estimation, and post-tree comparative analysis. The goal of this blog is to describe new tools we develop in Osiris and how to use them.